Biochemicals associated with Callosobruchus chinensis resistance in soybean

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Mercy‬ ‪Msiska Ulemu
Weldekidan Belay Miesho
Hailay Mehari Gebremedhn
Samuel Kyamanywa
Patrick Rubaihayo
Thomas Odong
Phinehas Tukamuhabwa
Ephraim Nuwamanya

Abstract

Soybean (Glycine max L.) is a very important crop worldwide because of its high protein and oil content. However, soybean suffers damage from bruchid (Callosobruchus chinensis) during storage. Understanding of factor(s) contributing to bruchid resistance is useful for development of soybean cultivars with genetic resistance to bruchid damage. Biochemicals associated with resistance to Callosobruchus chinensis were investigated on eight soybean genotypes with varying levels of resistance. Significant differences (P<0.05) were observed amongst genotypes with regards to antinutritional factors particularly total antioxidants (TAOX), tannins, peroxidase (POD), and flavonoids. There were no significant differences among genotypes with regards to nutritional factors particularly proteins, starch, lipid peroxidation, and reducing sugars. A resistant genotype AVRDC G8527 had the highest concentration of total antioxidants (1.98 AU min-1 mg-1) and tannins (1.85mg TAE 100g-1) followed by Maksoy 3N while the least was in a very susceptible genotype AGS 292 (TAOX=0.39 AU min-1 mg-1, Tannin=0.296 mg TAE 100g-1). AGS 292 had the highest concentration of flavonoids (31.22 mg QE 100g-1) while AVRDC G8527 had the least (5.13 mg QE 100g-1). Peroxidase activity was highest in AVRDC G89 (0.27AU min-1 mg-1) while AGS 292 had the least (0.07AU min-1 mg-1). From the correlation analysis, there was a significant strong negative relationship between flavonoids and tannin (r=-0.73**) and TAOX (r=- 0.71**) but a positive non-significant relationship with phenolic (r=0.22) and alkaloids (r=0.37) was recorded.

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